Rec8 Rabbit Polyclonal Antibody
Konjugasyon: Unconjugated
Rabbit polyclonal Antibody
Uygulama
Reaktivite
Human,Rat,Mouse
Gen Adı
REC8
Saklama
Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Özet
| Ürün Adı | Rec8 Rabbit Polyclonal Antibody |
| Açıklama | Rabbit polyclonal Antibody |
| Konak | Rabbit |
| Reaktivite | Human,Rat,Mouse |
| Konjugasyon | Unconjugated |
| Modifikasyon | Unmodified |
| İzotip | IgG |
| Klonalite | Polyclonal |
| Form | Liquid |
| Konsantrasyon | Unconjugated |
| Saklama | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
| Nakliye | Ice bags. |
| Tampon | Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% New type preservative N. |
| Saflaştırma | Affinity purification |
Antijen Bilgisi
| Gen Adı | REC8 |
| Alternatif İsimler | REC8; REC8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p |
| Gen Kimliği | 9985 |
| SwissProt Kimliği | O95072 |
| İmmünojen | The antiserum was produced against synthesized peptide derived from human REC8. AA range:154-203 |
Uygulama
| Uygulama | WB,IHC,ELISA |
| Seyreltme Oranı | WB 1:500-1:2000,IHC 1:50-1:300,ELISA 1:2000-1:20000 |
| Moleküler Ağırlık | 62kDa |
Araştırma Alanı
| Oocyte meiosis; |
Arka Plan
| This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008],function:Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.,PTM:Phosphorylated.,similarity:Belongs to the rad21 family.,subcellular location:In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.,subunit:Interacts in both phosphorylated and unphosphorylated forms with SMC3, SYCP3 and SMC1B but not with SMC1A. Also interacts with RAD51.,tissue specificity:Expressed in testis and thymus., |
