Keap1 Rabbit Polyclonal Antibody
Konjugasyon: Unconjugated
Rabbit polyclonal Antibody
Uygulama
Reaktivite
Human,Mouse,Rat
Gen Adı
KEAP1
Saklama
Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Özet
| Ürün Adı | Keap1 Rabbit Polyclonal Antibody |
| Açıklama | Rabbit polyclonal Antibody |
| Konak | Rabbit |
| Reaktivite | Human,Mouse,Rat |
| Konjugasyon | Unconjugated |
| Modifikasyon | Unmodified |
| İzotip | IgG |
| Klonalite | Polyclonal |
| Form | Liquid |
| Konsantrasyon | Unconjugated |
| Saklama | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
| Nakliye | Ice bags. |
| Tampon | Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% New type preservative N. |
| Saflaştırma | Affinity purification |
Antijen Bilgisi
| Gen Adı | KEAP1 |
| Alternatif İsimler | KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19 |
| Gen Kimliği | 9817 |
| SwissProt Kimliği | Q14145 |
| İmmünojen | The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460 |
Uygulama
| Uygulama | WB,IHC,ICC/IF,ELISA |
| Seyreltme Oranı | WB 1:500-1:2000,IHC 1:100-1:300,ICC/IF 1:50-1:200,ELISA 1:10000-1:20000 |
| Moleküler Ağırlık | 70kDa |
Araştırma Alanı
| Ubiquitin mediated proteolysis; |
Arka Plan
| This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],disease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degradation.,similarity:Contains 1 BACK (BTB/Kelch associated) domain.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Homodimer. Interacts with the N-terminal regulatory domain of NF2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Interacts with PGAM5.,tissue specificity:Broadly expressed, with highest levels in skeletal muscle., |
