COMT Rabbit Polyclonal Antibody
Konjugasyon: Unconjugated
Rabbit polyclonal Antibody
Uygulama
Reaktivite
Human,Rat,Mouse
Gen Adı
COMT
Saklama
Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Özet
| Ürün Adı | COMT Rabbit Polyclonal Antibody |
| Açıklama | Rabbit polyclonal Antibody |
| Konak | Rabbit |
| Reaktivite | Human,Rat,Mouse |
| Konjugasyon | Unconjugated |
| Modifikasyon | Unmodified |
| İzotip | IgG |
| Klonalite | Polyclonal |
| Form | Liquid |
| Konsantrasyon | Unconjugated |
| Saklama | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
| Nakliye | Ice bags. |
| Tampon | Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% New type preservative N. |
| Saflaştırma | Affinity purification |
Antijen Bilgisi
| Gen Adı | COMT |
| Alternatif İsimler | COMT; Catechol O-methyltransferase |
| Gen Kimliği | 1312 |
| SwissProt Kimliği | P21964 |
| İmmünojen | The antiserum was produced against synthesized peptide derived from human COMT. AA range:61-110 |
Uygulama
| Uygulama | WB,ELISA |
| Seyreltme Oranı | WB 1:500-1:2000,ELISA 1:10000-1:20000 |
| Moleküler Ağırlık | 30kDa |
Araştırma Alanı
| Steroid hormone biosynthesis;Tyrosine metabolism; |
Arka Plan
| Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008],catalytic activity:S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.,cofactor:Binds 1 magnesium ion per subunit.,function:Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.,mass spectrometry: PubMed:8020475,online information:Catechol-O-methyl transferase entry,polymorphism:Low enzyme activity alleles are associated with genetic susceptibility to alcoholism [MIM:103780].,polymorphism:Two alleles, COMT*1 or COMT*H with Val-158 and COMT*2 or COMT*L with Met-158 are responsible for a three to four-fold difference in enzymatic activity.,PTM:The N-terminus is blocked.,similarity:Belongs to the mammalian catechol-O-methyltransferase family.,tissue specificity:Brain, liver, placenta, lymphocytes and erythrocytes., |
