BOB1 (7F1) Rabbit Monoclonal Antibody-EnkiLife

BOB1 (7F1) Rabbit Monoclonal Antibody

Summary

Production Name	BOB1 (7F1) Rabbit Monoclonal Antibody
Description	Recombinant rabbit monoclonal antibody
Host	Rabbit
Application	WB,IHC,FC
Reactivity	Human

Performance

Conjugation	Unconjugated
Modification	Unmodified
Isotype	IgG
Clonality	Monoclonal
Form	Liquid
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification	Affinity purification

Immunogen

Gene Name	POU2AF1
Alternative Names	BOB 1; OBF1; OCAB; OCT binding factor 1; Pou2af1;
Gene ID	5450
SwissProt ID	Q16633

Application

Dilution Ratio	WB 1:2000-1:20000,IHC 1:100-1:200,FC 1:20-1:50
Molecular Weight	27kDa

Background

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).

Research Area

Immunology; Adaptive Immunity; B Cells; Non-CD; Epigenetics and Nuclear Signaling; Transcription; Domain Families; Developmental Families; POU