ARA54 Rabbit Polyclonal Antibody
Konjugasyon: Unconjugated
Rabbit polyclonal Antibody
Uygulama
Reaktivite
Human,Mouse,Rat
Gen Adı
RNF14
Saklama
Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Özet
| Ürün Adı | ARA54 Rabbit Polyclonal Antibody |
| Açıklama | Rabbit polyclonal Antibody |
| Konak | Rabbit |
| Reaktivite | Human,Mouse,Rat |
| Konjugasyon | Unconjugated |
| Modifikasyon | Unmodified |
| İzotip | IgG |
| Klonalite | Polyclonal |
| Form | Liquid |
| Konsantrasyon | Unconjugated |
| Saklama | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
| Nakliye | Ice bags. |
| Tampon | Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% New type preservative N. |
| Saflaştırma | Affinity purification |
Antijen Bilgisi
| Gen Adı | RNF14 |
| Alternatif İsimler | RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein |
| Gen Kimliği | 9604 |
| SwissProt Kimliği | Q9UBS8 |
| İmmünojen | The antiserum was produced against synthesized peptide derived from human RNF14. AA range:361-410 |
Uygulama
| Uygulama | WB,ELISA |
| Seyreltme Oranı | WB 1:500-1:2000,ELISA 1:20000-1:40000 |
| Moleküler Ağırlık | 50kDa |
Araştırma Alanı
Arka Plan
| The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011],caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,domain:The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.,domain:The RING-type zinc finger is essential for the interaction with UBE2E2.,function:Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.,pathway:Protein modification; protein ubiquitination.,PTM:RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.,similarity:Belongs to the RBR family. RNF14 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 1 RWD domain.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2 and in the presence of testosterone, with the androgen receptor (AR).,tissue specificity:Widely expressed., |
