ZAP70 Mouse Monoclonal Antibody
Konjugasyon: Unconjugated
Mouse monoclonal Antibody
Uygulama
Reaktivite
Human
Gen Adı
ZAP70
Saklama
Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Özet
| Ürün Adı | ZAP70 Mouse Monoclonal Antibody |
| Açıklama | Mouse monoclonal Antibody |
| Konak | Mouse |
| Reaktivite | Human |
| Konjugasyon | Unconjugated |
| Modifikasyon | Unmodified |
| İzotip | Mouse IgG1 |
| Klonalite | Monoclonal |
| Form | Liquid |
| Konsantrasyon | Unconjugated |
| Saklama | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
| Nakliye | Ice bags. |
| Tampon | Purified antibody in PBS with 0.05% sodium azide. |
| Saflaştırma | Affinity Purification |
Antijen Bilgisi
| Gen Adı | ZAP70 |
| Alternatif İsimler | Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK |
| Gen Kimliği | 7535 |
| SwissProt Kimliği | P43403 |
| İmmünojen | This ZAP70 antibody is generated from a mouse immunized with a recombinant protein. |
Uygulama
| Uygulama | WB,ICC,FC |
| Seyreltme Oranı | WB 1:2000-1:4000,ICC 1:25-1:50,FC 1:25-1:50 |
| Moleküler Ağırlık | 69.9kDa |
Araştırma Alanı
Arka Plan
| Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR). |
