WT1 Mouse Monoclonal Antibody
Konjugasyon: Unconjugated
Mouse monoclonal Antibody
Uygulama
Reaktivite
Human, Monkey
Gen Adı
WT1
Saklama
Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Özet
| Ürün Adı | WT1 Mouse Monoclonal Antibody |
| Açıklama | Mouse monoclonal Antibody |
| Konak | Mouse |
| Reaktivite | Human, Monkey |
| Konjugasyon | Unconjugated |
| Modifikasyon | Unmodified |
| İzotip | Mouse IgG1 |
| Klonalite | Monoclonal |
| Form | Liquid |
| Konsantrasyon | Unconjugated |
| Saklama | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
| Nakliye | Ice bags. |
| Tampon | Purified antibody in PBS with 0.05% sodium azide |
| Saflaştırma | Affinity Purification |
Antijen Bilgisi
| Gen Adı | WT1 |
| Alternatif İsimler | GUD; AWT1; WAGR; WT33; NPHS4; WIT-2 |
| Gen Kimliği | 7490 |
| SwissProt Kimliği | P19544 |
| İmmünojen | Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli. |
Uygulama
| Uygulama | WB,IHC,ICC,ELISA,FC |
| Seyreltme Oranı | WB 1:500-1:2000,IHC 1:200-1:1000,ICC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400 |
| Moleküler Ağırlık | 49.2kDa |
Araştırma Alanı
Arka Plan
| This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. |
