Smad2/3 Mouse Monoclonal Antibody
Konjugasyon: Unconjugated
Mouse monoclonal Antibody
Uygulama
Reaktivite
Human,Mouse,Rat
Gen Adı
Smad2/3
Saklama
Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Özet
| Ürün Adı | Smad2/3 Mouse Monoclonal Antibody |
| Açıklama | Mouse monoclonal Antibody |
| Konak | Mouse |
| Reaktivite | Human,Mouse,Rat |
| Konjugasyon | Unconjugated |
| Modifikasyon | Unmodified |
| İzotip | Mouse IgG |
| Klonalite | Monoclonal |
| Form | Liquid |
| Konsantrasyon | Unconjugated |
| Saklama | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
| Nakliye | Ice bags. |
| Tampon | Purified antibody in PBS with 0.05% sodium azide,0.5%protective protein and 50% glycerol. |
| Saflaştırma | Affinity Purification |
Antijen Bilgisi
| Gen Adı | Smad2/3 |
| Alternatif İsimler | SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 |
| Gen Kimliği | 4087/4088 |
| SwissProt Kimliği | P84022/Q15796 |
| İmmünojen | Recombinant Protein of Smad3 |
Uygulama
| Uygulama | WB,IHC |
| Seyreltme Oranı | WB 1:500-1:1000,IHC 1:50-1:100 |
| Moleküler Ağırlık | Calculated MW: 52 kDa; Observed MW: 52,60 kDa |
Araştırma Alanı
Arka Plan
| Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses . |
