Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF‑β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor‑regulated SMADs (R‑SMADs), which include SMAD1, 2, 3, 5, and 9; the common‑mediator SMAD (co‑SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I‑SMADs), SMAD6 and 7. Activated type I receptors associate with specific R‑SMADs and phosphorylate them on a conserved carboxy‑terminal SSXS motif. The phosphorylated R‑SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity.
| Target | Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|---|
| Smad1/5/9 | APRab17987 | Smad1/5/9 Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Smad1 | APRab17986 | Smad1 Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad1 (Ser465) | APRab05437 | Phospho‑Smad1 (Ser465) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad1 (Ser187) | APRab05436 | Phospho‑Smad1 (Ser187) Rabbit Polyclonal Antibody | Human,Mouse,Monkey | WB,IHC,ICC/IF,ELISA |
| Smad2/3 | AMRe87734 | Smad2/3 Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,ICC/IF,FC,IP |
| Smad2 | AMRe21514 | Smad2 Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,ICC/IF,ELISA,IP |
| Phospho‑Smad2/3 (Thr8) | AMRe21074 | Phospho‑Smad2/3 (Thr8) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,ICC/IF,ELISA,IP |
| Phospho‑Smad2 (Thr220) | APRab05442 | Phospho‑Smad2 (Thr220) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad2 (Ser467) | APRab05441 | Phospho‑Smad2 (Ser467) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad2 (Ser250) | AMRe21461 | Phospho‑Smad2 (Ser250) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IHC,IF,ELISA |
| Acetyl‑Smad2/3 (Acetyl‑Lys19) | APRab06256 | Acetyl‑Smad2/3 (Acetyl‑Lys19) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF |
| Smad3 | APRab17994 | Smad3 Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Smad3 | AMRe02613 | Smad3 Rabbit Monoclonal Antibody | Human,Mouse,Rat,Hamster | WB,IP |
| Phospho‑Smad3 (Ser423/425) | AMRe06012 | Phospho‑Smad3 (Ser423/425) (12O16) Rabbit Monoclonal Antibody | Human,Mouse | WB,IHC,ICC/IF |
| Phospho‑Smad3 (Ser425) | APRab00823 | Phospho‑Smad3 (Ser425) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad2 (Thr8)/Smad3 (Thr8) | AMRe87735 | Phospho‑Smad2 (Thr8)/Smad3 (Thr8) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,ICC/IF,IP |
| Phospho‑Smad3 (Thr179) | APRab05449 | Phospho‑Smad3 (Thr179) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad3 (Ser213) | APRab05447 | Phospho‑Smad3 (Ser213) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad3 (Ser208) | APRab05446 | Phospho‑Smad3 (Ser208) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Phospho‑Smad3 (Ser204) | APRab05445 | Phospho‑Smad3 (Ser204) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA |
| Smad4 | AMRe03205 | Smad4 Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IHC,IF,IP,ELISA |
| Smad5 | AMRe21160 | Smad5 Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA,IP |
| Phospho‑SMAD5 (Ser463/Ser465) | AMRe21103 | Phospho‑SMAD5 (Ser463/Ser465) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IHC,ICC/IF,ELISA,IP |
| Smad6 | APRab18000 | Smad6 Rabbit Polyclonal Antibody | Human,Mouse | WB,IHC,ICC/IF,ELISA |
Related Products
Super-sensitive ECL chemiluminescent reagent
References
- TGF‑beta signalling from cell membrane to nucleus through SMAD proteins. Heldin CH, et al. Nature. 1997. [PMID: 9393997]
- Mads and Smads in TGF beta signalling. Attisano L, et al. Curr Opin Cell Biol. 1998. [PMID: 9561843]
- Smads: transcriptional activators of TGF‑beta responses. Derynck R, et al. Cell. 1998. [PMID: 9865691]
- Smads: transcriptional activators of TGF‑beta responses. Derynck R, et al. Cell. 1998. [PMID: 9865691]
- TGF‑beta signal transduction. Massagué J. Annu Rev Biochem. 1998. [PMID: 9759503]
- Smads and early developmental signaling by the TGFbeta superfamily. Whitman M. Genes Dev. 1998. [PMID: 9716398]
- Crossing Smads. Wrana JL. Sci STKE. 2000. [PMID: 11752591]
- Signal transduction by the TGF‑beta superfamily. Attisano L, et al. Science. 2002. [PMID: 12040180]
- Smad regulation in TGF‑beta signal transduction. Moustakas A, et al. J Cell Sci. 2001. [PMID: 11792802]
